FLIM & Advanced Fluorescence
Fluorescence lifetime imaging and advanced fluorescence techniques
Go beyond intensity-based fluorescence with FLIM (Fluorescence Lifetime Imaging Microscopy) on the openUC2 platform. Measure fluorophore lifetimes to distinguish molecular environments, detect FRET, and separate overlapping emission spectra. The modular architecture lets you add pulsed laser excitation and time-correlated detection to any existing UC2 setup.
Technical specifications
| Excitation | Pulsed laser diode (405/470/640 nm) |
|---|---|
| Detection | SPAD or hybrid PMT module (UC2 cube) |
| Timing electronics | TCSPC module (PicoQuant compatible) |
| Lifetime range | 100 ps to 100 ns |
| Spatial resolution | Diffraction-limited |
| Software | ImSwitch + FLIM analysis plugin |
Key capabilities
Modular FLIM upgrade
Add pulsed laser and TCSPC detection to your existing UC2 fluorescence setup.
FRET detection
Measure donor-acceptor distances via fluorescence lifetime changes.
Environmental sensing
Map pH, viscosity, and ion concentrations via lifetime-sensitive probes.
Open data format
Export lifetime data in standard formats for analysis in FLIMfit, LLSpy, or custom scripts.
On the roadmap
Interested in flim & advanced fluorescence?
Tell us about your imaging needs and we'll help you find the right configuration.